p erk (Cell Signaling Technology Inc)
99
Structured Review
Cell Signaling Technology Inc
p erk
P Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 13375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+phospho+erk/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+XP+Rabbit+mAb/pmc13042271-96-58-63
Average 99 stars, based on 13375 article reviews
P Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 13375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+phospho+erk/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+XP+Rabbit+mAb/pmc13042271-96-58-63
Average 99 stars, based on 13375 article reviews
p erk - by Bioz Stars,
2026-09
99/100 stars
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Western Blot:Article Title: MYC-Driven Activation of USP39 Enhances SRSF1 Stability and Promotes PDAC Progression Article Snippet: Cells were harvested and lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4; 150 mM NaCl; 0.1% SDS; 1% NP-40; 0.5% sodium deoxycholate; 1 mM PMSF; and protease inhibitor cocktail [Roche]) by incubating on ice for 30 min. For protein stability and half-life analysis, cells were treated with 10 μg/mL cycloheximide (Sigma-Aldrich) for the indicated time points. .. Western blotting was performed using the following primary antibodies at 1:1000 dilution: rabbit anti- SRSF1 (Abcam), rabbit anti-USP39 (Cell Signaling Technology), rabbit anti-Tubulin (Proteintech), rabbit anti-T7 (Proteintech), rabbit anti-ERK (Cell Signaling Technology), Article Title: FGF receptor modulates planar cell polarity in the neuroectoderm via Vangl2 tyrosine phosphorylation Article Snippet: .. Immunoblotting was carried out with goat anti-biotin-HRP antibodies (Cell Signaling) or goat anti-biotin antibody (Pierce), anti-FLAG (M2, Millipore Sigma), rabbit anti-HA (Bethyl) antibodies, mouse anti-HA (12A5), Nucleic Acid Electrophoresis:Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome. Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), Membrane:Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome. Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), Incubation:Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome. Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain Article Snippet: .. After blocking with fat-free milk (5%), the membranes were incubated overnight at 4°C with the following primary antibodies: rabbit anti-PRMT6 (1:1000; Novus Biologicals, Cat# NB100-56642, RRID: AB_838734), mouse anti-hnRNP-F (1:1000; Thermo Fisher Scientific, Cat# MA5-18024, RRID: AB_2539408), rabbit anti-MOR (1:1000; Abclonal, Cat# A16939, RRID: AB_2770729), rabbit anti-asymmetric dimethylation of histone H3 arginine 2 (H3R2me2a; 1:1000; Abclonal, Cat# A3155, RRID: AB_2764949), rabbit anti-ERK (1:1000; Cell Signaling Technology, Danvers, MA, USA, Cat# 4695S, RRID: AB_390779), Blocking Assay:Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain Article Snippet: .. After blocking with fat-free milk (5%), the membranes were incubated overnight at 4°C with the following primary antibodies: rabbit anti-PRMT6 (1:1000; Novus Biologicals, Cat# NB100-56642, RRID: AB_838734), mouse anti-hnRNP-F (1:1000; Thermo Fisher Scientific, Cat# MA5-18024, RRID: AB_2539408), rabbit anti-MOR (1:1000; Abclonal, Cat# A16939, RRID: AB_2770729), rabbit anti-asymmetric dimethylation of histone H3 arginine 2 (H3R2me2a; 1:1000; Abclonal, Cat# A3155, RRID: AB_2764949), rabbit anti-ERK (1:1000; Cell Signaling Technology, Danvers, MA, USA, Cat# 4695S, RRID: AB_390779), |