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p erk  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p erk
    P Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 13375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+erk/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+XP+Rabbit+mAb/pmc13042271-96-58-63
    Average 99 stars, based on 13375 article reviews
    p erk - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: MYC-Driven Activation of USP39 Enhances SRSF1 Stability and Promotes PDAC Progression
    Article Snippet: Cells were harvested and lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4; 150 mM NaCl; 0.1% SDS; 1% NP-40; 0.5% sodium deoxycholate; 1 mM PMSF; and protease inhibitor cocktail [Roche]) by incubating on ice for 30 min. For protein stability and half-life analysis, cells were treated with 10 μg/mL cycloheximide (Sigma-Aldrich) for the indicated time points. .. Western blotting was performed using the following primary antibodies at 1:1000 dilution: rabbit anti- SRSF1 (Abcam), rabbit anti-USP39 (Cell Signaling Technology), rabbit anti-Tubulin (Proteintech), rabbit anti-T7 (Proteintech), rabbit anti-ERK (Cell Signaling Technology), rabbit anti-phospho-ERK (Cell Signaling Technology), rabbit anti-MYC (Cell Signaling Technology), rabbit anti-HA (Proteintech), and mouse anti-Flag (MilliporeSigma). .. Blots were imaged and quantified using the Odyssey Infrared Imaging System (LI-COR).

    Article Title: FGF receptor modulates planar cell polarity in the neuroectoderm via Vangl2 tyrosine phosphorylation
    Article Snippet: .. Immunoblotting was carried out with goat anti-biotin-HRP antibodies (Cell Signaling) or goat anti-biotin antibody (Pierce), anti-FLAG (M2, Millipore Sigma), rabbit anti-HA (Bethyl) antibodies, mouse anti-HA (12A5), rabbit anti-phospho-ERK (Cell Signaling), rabbit anti-ERK (Cell Signaling), mouse anti-Vangl2 (C8, Santa Cruz), rat monoclonal anti-Vangl2 (2G4, Millipore Sigma), and mouse anti-GFP (B2, Santa Cruz). .. Chemiluminescence was captured by ChemiDoc (BioRad), and the Biotin/HA chemiluminescence intensity ratios were quantified using the ChemiDoc software.

    Nucleic Acid Electrophoresis:

    Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome.
    Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), rabbit anti-phospho-ERK (1:1000, 4370, D13.14.4E clone, CST), rabbit anti-JNK (1:1000, 9258, 56G8 clone, CST), rabbit anti-phospho-JNK (1:1000, 4668, 81E11 clone, CST), rabbit anti-p38 (1:1000, 8690, D13E1 clone, CST), rabbit anti-phospho-p38 (1:1000, 4511, D3F9 clone, CST), rabbit anti-NLRP3 (1:1000, 15101, D4D8T clone, CST), rabbit anti-AKT (1:1000, C67E7 clone, 4691, CST), rabbit anti-phospho-AKT (1:1000, C31E5E clone, 2965, CST), mouse anti-Caspase-1 (1:1000, AG-20B-0042, Adipogen), and rabbit anti-IL1 beta (1:1000, 5128, Biovision), rabbit anti-Ubiquitin (1:500, GB115700, ServiceBio) overnight at 4 ◦ C. After incubation with peroxidase-conjugated secondary antibodies, the signals were detected by ECL assays (WBKLS0500, Millipore, USA). .. Anti-GAPDH (1:1000, AF1186, Beyotime, China) or β-actin antibody (1:1000, 3700, CST) was used as an internal control for total cytoplasmic protein, and histone H3 (1:300, GB11102, ServiceBio) were used as an internal control for nuclear protein.

    Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome
    Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), rabbit anti-phospho-ERK (1:1000, 4370, D13.14.4E clone, CST), rabbit anti-JNK (1:1000, 9258, 56G8 clone, CST), rabbit anti-phospho-JNK (1:1000, 4668, 81E11 clone, CST), rabbit anti-p38 (1:1000, 8690, D13E1 clone, CST), rabbit anti-phospho-p38 (1:1000, 4511, D3F9 clone, CST), rabbit anti-NLRP3 (1:1000, 15101, D4D8T clone, CST), rabbit anti-AKT (1:1000, C67E7 clone, 4691, CST), rabbit anti-phospho-AKT (1:1000, C31E5E clone, 2965, CST), mouse anti-Caspase-1 (1:1000, AG-20B-0042, Adipogen), and rabbit anti-IL-1 beta (1:1000, 5128, Biovision), rabbit anti-Ubiquitin (1:500, GB115700 , ServiceBio) overnight at 4°C. .. After incubation with peroxidase-conjugated secondary antibodies, the signals were detected by ECL assays (WBKLS0500, Millipore, USA).

    Membrane:

    Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome.
    Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), rabbit anti-phospho-ERK (1:1000, 4370, D13.14.4E clone, CST), rabbit anti-JNK (1:1000, 9258, 56G8 clone, CST), rabbit anti-phospho-JNK (1:1000, 4668, 81E11 clone, CST), rabbit anti-p38 (1:1000, 8690, D13E1 clone, CST), rabbit anti-phospho-p38 (1:1000, 4511, D3F9 clone, CST), rabbit anti-NLRP3 (1:1000, 15101, D4D8T clone, CST), rabbit anti-AKT (1:1000, C67E7 clone, 4691, CST), rabbit anti-phospho-AKT (1:1000, C31E5E clone, 2965, CST), mouse anti-Caspase-1 (1:1000, AG-20B-0042, Adipogen), and rabbit anti-IL1 beta (1:1000, 5128, Biovision), rabbit anti-Ubiquitin (1:500, GB115700, ServiceBio) overnight at 4 ◦ C. After incubation with peroxidase-conjugated secondary antibodies, the signals were detected by ECL assays (WBKLS0500, Millipore, USA). .. Anti-GAPDH (1:1000, AF1186, Beyotime, China) or β-actin antibody (1:1000, 3700, CST) was used as an internal control for total cytoplasmic protein, and histone H3 (1:300, GB11102, ServiceBio) were used as an internal control for nuclear protein.

    Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome
    Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), rabbit anti-phospho-ERK (1:1000, 4370, D13.14.4E clone, CST), rabbit anti-JNK (1:1000, 9258, 56G8 clone, CST), rabbit anti-phospho-JNK (1:1000, 4668, 81E11 clone, CST), rabbit anti-p38 (1:1000, 8690, D13E1 clone, CST), rabbit anti-phospho-p38 (1:1000, 4511, D3F9 clone, CST), rabbit anti-NLRP3 (1:1000, 15101, D4D8T clone, CST), rabbit anti-AKT (1:1000, C67E7 clone, 4691, CST), rabbit anti-phospho-AKT (1:1000, C31E5E clone, 2965, CST), mouse anti-Caspase-1 (1:1000, AG-20B-0042, Adipogen), and rabbit anti-IL-1 beta (1:1000, 5128, Biovision), rabbit anti-Ubiquitin (1:500, GB115700 , ServiceBio) overnight at 4°C. .. After incubation with peroxidase-conjugated secondary antibodies, the signals were detected by ECL assays (WBKLS0500, Millipore, USA).

    Incubation:

    Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome.
    Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), rabbit anti-phospho-ERK (1:1000, 4370, D13.14.4E clone, CST), rabbit anti-JNK (1:1000, 9258, 56G8 clone, CST), rabbit anti-phospho-JNK (1:1000, 4668, 81E11 clone, CST), rabbit anti-p38 (1:1000, 8690, D13E1 clone, CST), rabbit anti-phospho-p38 (1:1000, 4511, D3F9 clone, CST), rabbit anti-NLRP3 (1:1000, 15101, D4D8T clone, CST), rabbit anti-AKT (1:1000, C67E7 clone, 4691, CST), rabbit anti-phospho-AKT (1:1000, C31E5E clone, 2965, CST), mouse anti-Caspase-1 (1:1000, AG-20B-0042, Adipogen), and rabbit anti-IL1 beta (1:1000, 5128, Biovision), rabbit anti-Ubiquitin (1:500, GB115700, ServiceBio) overnight at 4 ◦ C. After incubation with peroxidase-conjugated secondary antibodies, the signals were detected by ECL assays (WBKLS0500, Millipore, USA). .. Anti-GAPDH (1:1000, AF1186, Beyotime, China) or β-actin antibody (1:1000, 3700, CST) was used as an internal control for total cytoplasmic protein, and histone H3 (1:300, GB11102, ServiceBio) were used as an internal control for nuclear protein.

    Article Title: TLR9 decreases FOXO3 expression to prevent excessive inflammation in macrophage activation syndrome
    Article Snippet: For the localization detection of FOXO3, cytoplasmic and nuclear proteins were extracted from cells using a protein extraction kit (KeyGen BioTECH, KGB5300). .. Proteins in cell lysates were first resolved using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane, and subsequently incubated with the primary antibodies against rabbit anti-FOXO3a (1:1000, 12829, D19A7 clone, CST), rabbit anti-phospho-FoxO3a Ser253 (1:1000, 13129, D18H8 clone, CST), rabbit anti-IRF7 (1:1000, 4920, CST), rabbit anti-phospho-IRF7 (1:1000, 5184, CST), rabbit anti-ERK (1:1000, 4695, 137F5 clone, CST), rabbit anti-phospho-ERK (1:1000, 4370, D13.14.4E clone, CST), rabbit anti-JNK (1:1000, 9258, 56G8 clone, CST), rabbit anti-phospho-JNK (1:1000, 4668, 81E11 clone, CST), rabbit anti-p38 (1:1000, 8690, D13E1 clone, CST), rabbit anti-phospho-p38 (1:1000, 4511, D3F9 clone, CST), rabbit anti-NLRP3 (1:1000, 15101, D4D8T clone, CST), rabbit anti-AKT (1:1000, C67E7 clone, 4691, CST), rabbit anti-phospho-AKT (1:1000, C31E5E clone, 2965, CST), mouse anti-Caspase-1 (1:1000, AG-20B-0042, Adipogen), and rabbit anti-IL-1 beta (1:1000, 5128, Biovision), rabbit anti-Ubiquitin (1:500, GB115700 , ServiceBio) overnight at 4°C. .. After incubation with peroxidase-conjugated secondary antibodies, the signals were detected by ECL assays (WBKLS0500, Millipore, USA).

    Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain
    Article Snippet: .. After blocking with fat-free milk (5%), the membranes were incubated overnight at 4°C with the following primary antibodies: rabbit anti-PRMT6 (1:1000; Novus Biologicals, Cat# NB100-56642, RRID: AB_838734), mouse anti-hnRNP-F (1:1000; Thermo Fisher Scientific, Cat# MA5-18024, RRID: AB_2539408), rabbit anti-MOR (1:1000; Abclonal, Cat# A16939, RRID: AB_2770729), rabbit anti-asymmetric dimethylation of histone H3 arginine 2 (H3R2me2a; 1:1000; Abclonal, Cat# A3155, RRID: AB_2764949), rabbit anti-ERK (1:1000; Cell Signaling Technology, Danvers, MA, USA, Cat# 4695S, RRID: AB_390779), rabbit anti-phospho-ERK (1:1000; Cell Signaling Technology, Cat# 4377, RRID: AB_331775), and rabbit anti-H3 (1:2000; Cell Signaling Technology, Cat# 4499, RRID: AB_10544537). .. Finally, the membranes were cinubatee with an anti-rabbit secondary antibody (1:5000) conjugated with horseradish peroxidase (BBI, Nashville, TN, USA, Cat# D110058) and an anti-mouse secondary antibody (1:5000; BBI, Cat# D110085) for 2 hours at room temperature.

    Blocking Assay:

    Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain
    Article Snippet: .. After blocking with fat-free milk (5%), the membranes were incubated overnight at 4°C with the following primary antibodies: rabbit anti-PRMT6 (1:1000; Novus Biologicals, Cat# NB100-56642, RRID: AB_838734), mouse anti-hnRNP-F (1:1000; Thermo Fisher Scientific, Cat# MA5-18024, RRID: AB_2539408), rabbit anti-MOR (1:1000; Abclonal, Cat# A16939, RRID: AB_2770729), rabbit anti-asymmetric dimethylation of histone H3 arginine 2 (H3R2me2a; 1:1000; Abclonal, Cat# A3155, RRID: AB_2764949), rabbit anti-ERK (1:1000; Cell Signaling Technology, Danvers, MA, USA, Cat# 4695S, RRID: AB_390779), rabbit anti-phospho-ERK (1:1000; Cell Signaling Technology, Cat# 4377, RRID: AB_331775), and rabbit anti-H3 (1:2000; Cell Signaling Technology, Cat# 4499, RRID: AB_10544537). .. Finally, the membranes were cinubatee with an anti-rabbit secondary antibody (1:5000) conjugated with horseradish peroxidase (BBI, Nashville, TN, USA, Cat# D110058) and an anti-mouse secondary antibody (1:5000; BBI, Cat# D110085) for 2 hours at room temperature.



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